Internal validation of latest generation STR kits for direct STR typing from reference samples
نویسندگان
چکیده
منابع مشابه
Next-Generation STR Genotyping Kits for Forensic Applications.
Forensic DNA typing has been a constantly evolving field driven by innovations from academic laboratories as well as kit manufacturers. Central to these technological advances has been the transition from multilocus-probe restriction fragment length polymorphism (RFLP) methods to short tandem repeat (STR) PCR-based assays. STRs are now the markers of choice for forensic DNA typing and a wide va...
متن کاملValidation Studies on STR Systems
Before a new STR system or STR multiplex may be routinely employed in human identity testing it should be extensively validated to insure reliability of results. We have included below many of the validation studies which have appeared in the literature. We hope this material will help future researchers design their validation studies and allow those who use common STR systems to review what s...
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Human fecal matter is routinely encountered in forensic casework—identifying the source of this material could link a suspect or victim to a crime scene. Previous methods used to identify the source of the fecal matter have primarily involved microscopic comparisons. While these methods can be useful, two sources of fecal matter are required, and typically the analysis cannot individualize the ...
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Whole blood from various individuals was spotted on 100% white cotton fabric. Once the bloodstains were dry, approximately 1 mm or less of the bloodstained thread was cut from each sample and placed in an autoclaved reaction tube containing either TE buffer or sterile distilled water. The tubes were then subjected to a simple preincubation period as described by the author previously (1). After...
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Human identification from DNA is typically based on 13 short-tandem repeat (STR) alleles. Commercial kits used in forensic casework rely on the detection of these alleles in DNA samples acquired from an individual. However, the process itself is slow (it can take up to 2 days when conducting a laboratory analysis or 1 hour when using Rapid DNA systems) and has been designed to operate on pristi...
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ژورنال
عنوان ژورنال: Forensic Science International: Genetics Supplement Series
سال: 2019
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2019.10.194